Polymerase chain reaction (PCR) is based on the amplification of specific regions of the pathogen genome. In real-time PCR, the amplified product is detected using fluorescent dyes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows detection of the accumulating product without the need to re-open the reaction tubes afterward. The -ASFV PCR Kit contains all of the necessary reagents for the detection of ASFV DNA, including reagents for Express DNA purification, external, positive and negative controls. An external control excludes the possibility of false-negative results.
The kit uses three specific primer/probe combinations: Two – for ASFV DNA yielding HEX™ fluorescence and one for an external control yielding FAM™ fluorescence. A PC serves to verify the functionality of the reaction mixture for the amplification of the ASFV DNA target.