YZZY Start DNA polymerase is a high-quality hot-start polymerase with superior sensitivity and specificity.
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Area of applications
• Routine PCR
• Multiplex PCR
• Low-copy PCR
• Highly specific PCR
• PCR with dual labeled probes
• Real-time PCR with intercalating dyes
Benefits
• Increased sensitivity and specifity
• Room temperature storage up to 1 month
• Storage without freezing (2-8°C for 6 month)
• DNA fragments length up to 5 kbp
Size, U | Components* |
1 000 | 0.2 mL – Y-Start, 5 U/μL |
5 000 | 1 mL – Y-Start, 5 U/μL |
50 000 | 10 mL – Y-Start, 5 U/μL |
500 000 | 2×50 mL – Y-Start, 5 U/μL |
* Can be supplied with original 10x PCR buffer and 50 mM MgCl2 upon request
The reagent is composed of a high-purified recombinant Taq DNA polymerase
and specific aptamer which inhibits enzymatic activity of polymerase at room
temperature. According to our tests, 1 Unit of polymerase keeps inactive during the
incubation with the substrate for about 0.5 hour at 37°C. Activation of the enzyme
starts automatically during the first cycle of DNA denaturation.
Due to original storage buffer the reagent can be stored at room temperature
(25°C) for up to 1 month or at 2-8°C for 6 months without significant changes in
activity and inactivation level.