YZZY MMLV
YZZY MMLV is a genetically modified reverse transcriptase of Moloney Mouse Leukemia Virus (MMLV) which performs the synthesis of a complementary strand of DNA (cDNA) on a single-stranded RNA template.
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Characteristics
Area of applications
• Synthesys of cDNA
• RNA analysis by primer extension
• Preparation cDNA libraries
• One-step RT-PCR
• Incorporation of labeled nucleotides
Benefites
• Efficient cDNA synthesis
• Increased thermostability (remain active
below 65°C)
• Inhibited RNase H activity
• Long fragments
amplification (up to 12 kbp)
• Increased productivity with GC-rich RNA template
| Size, U | Components |
| 20 000 | 0.1 mL – Y-MMLV, 200 U/μL |
| 100 000 | 0.5 mL – Y-MMLV, 200 U/μL |
| 200 000 | 1 mL – Y-MMLV, 200 U/μL |
| 1 000 000 | 5 mL – Y-MMLV, 200 U/μL |
* Supplied with original 10x buffer and 50 mM MgCl2
YZZY MMLV is a genetically modified reverse transcriptase of Moloney Mouse Leukemia
Virus (MMLV) which performs the synthesis of a complementary strand of DNA
(cDNA) on a single-stranded RNA template. Due to the presence of several
mutations, the enzyme possesses inhibited RNase H activity and has increased
thermostability at below 65°C, with an optimal temperature of 50°C.
Compared to wild-type MMLV, our enzyme provides higher cDNA yield, allows
longer long (up to 12 kbp) fragments amplification and has higher productivity
with GC-rich RNA templates.
