YZZY 5x Probe qPCR-mastermix

5x Fast Probe qPCR Mastermix is a universal ready-to-use reagent for quantitative and/or qualitative qPCR assay of plasmid, genomic and viral DNA.

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Characteristics

Area of applications
• Multiplex PCR
• qPCR with dual-labled probes

Benefits
• High sensitivity (single copies of DNA)
• High multiplex PCR performance (up to 4 reactions)
• Increased inhibition resistance
• Up to 6 month storage at 2-8°C

 

Size, reactions,
(25 μL each)
Components* 
10 000  50 mL – 5x qPCR 
50 000  5×50 mL – 5x qPCR 

* Can be supplied with ROX reference dye

5x Fast-probe qRCR Mastermix performance in a rapid cycling qPCR assay of GMI standarts titiration (form 5 to 50 000 copies in a reaction)

Description

5x Fast Probe qPCR Mastermix is a universal ready-to-use reagent for quantitative and/or qualitative qPCR assay of plasmid, genomic and viral DNA.
The Mastermix contains all basic components, including a thermostable DNA polymerase with a “hot start”, dNTPs, an optimized buffer with an enhancer, a stabilizer, MgCl2 (final concentration is 5 mM) and optional ROX reference dye (final concentration is 0.5 or 0.03 μM).
5x Fast Probe qPCR Mastermix provides reproducible results with high sensitivity (single copies of DNA) and has high multiplex PCR performance (up to 4 reactions). The reagent has increased inhibition resistivity.
5x Fast Probe qPCR Mastermix can be stored at temperatures up to 2-8°C for 6
month without loss of activity and specificity.