YZZY 5x One-step qPCR Mastermix

YZZY 5x One-Step qPCR Mastermix is a universal ready-to-use reagent for the synthesis of cDNA followed by quantitative and/or qualitative PCR with real-time detection of the results.

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Characteristics

Area of applications
• Multiple RT-PCR
• Routine RT-PCR
• Low-copy RT-PCR
• RT-PCR with dual-labeled probes


Benefites
• High sensitivity (single copies of RNA)
• High multiplex RT-PCR performance (up to 4 reactions)
• Increased inhibition resistance
• Up to 3 month storage at 2-8°C

 

Size, reactions,
(25 μL each)
Components 
1000  4×1.25 mL – 5x RT-PCR 
5000  20×1.25 mL – 5x RT-PCR 

Description

YZZY 5x One-Step qPCR Mastermix is a universal ready-to-use reagent for the synthesis of cDNA followed by quantitative and/or qualitative PCR with real-time detection of the results. The synthesis of cDNA and qPCR is carried out in a single tube. The Mastermix contains all basic components, including a revertase with a “warm start”, a thermostable DNA polymerase with a “hot start”, dNTPs and an optimized buffer with an enhancer and a stabilizer.
YZZY 5x One-Step qPCR Mastermix Mastermix provides reproducible results with high sensitivity
(single copies of RNA) and has high multiplex RT-PCR performance (up to 4 reactions).
YZZY 5x One-Step qPCR Mastermix can be stored at temperatures up to 2-8°C for 90 days without loss of activity and specificity.

YZZY 5x One-Step qPCR Mastermix performance in RT-PCR assay of GAPDH standarts titration (form 5 to 5×109 copies in a reaction)